Associate Professor  
Department of Internal Medicine, Biomedical Engineering

Ph.D., Chemistry/Spectroscopy, Indian Institute of Technology, Chennai, India, 1985

Research 
Division :

Biological Spectroscopy and Imaging 

Contact :

Tzagournis Medical Research Facility, Room 114  
420 West 12th Ave  
Columbus, OH 43210  
(614) 292-8998

kuppusamy-1@medctr.osu.edu

http://heartlung.osu.edu/2418.cfm?id=kuppusamy.1  

Biosketch  

Students :

Simi-Mary Chacko , Aditi Kulkarni , Tennille Presley , Deepti Vikram  

Alumni :

Li Haihong  

Dr. Kuppusamy received his doctoral (Ph.D.) degree in 1986 with specialization in EPR spectroscopy from Indian Institute of Technology, Madras, India. Following a Fogarty Fellowship from NIH, Dr. Kuppusamy joined the Johns Hopkins University School of Medicine in 1987 as a Research Fellow in the Division of Cardiology and moved to the rank of Associate Professor in 2001. In July 2002 he moved to the Ohio State University, where he is currently an Associate Professor in the Department of Internal Medicine. He also serves as the Director of the Biomedical EPR Spectroscopy & Imaging Facility in the HLRI.

Dr. Kuppusamy is uniquely talented with biophysical and mathematical concepts, computer software interfacing and programming expertise related to biomedical magnetic resonance spectroscopy and imaging. He has made significant contributions to the development of low-frequency electron paramagnetic resonance (EPR) spectroscopy and imaging methods for detecting free radicals (paramagnetic molecules possessing unpaired electrons) in biological systems. With Dr. Jay L. Zweier, he has developed a variety of in vivo EPR spectroscopic and imaging techniques enabling noninvasive measurements of free radicals and oxygen in intact biological tissues, isolated organs, and small animals. This development will have major influence in the study of free radical-mediated pathophysiology and pave the way for functional EPR imaging of living systems, including humans.

The development of magnetic resonance techniques to image physiologically pertinent (functional) parameters such as oxygenation and energy metabolism in myocardium or tumor tissue is an important part of his commitment at the EPR Facility.

Kuppusamy, P., Li, H., Ilangovan, G., Cardounel, A. J., Zweier, J. L., Yamada, K., Krishna, M. C., and Mitchell, J. B. Noninvasive imaging of tumor redox status and its modification by tissue glutathione levels. Cancer Research, 62, 307-312 (2002).

Ilangovan, G., Manivannan, A., Li, H., Yanagi, H., Zweier, J. L., and Kuppusamy, P. A naphthalocyanine-based EPR probe for localized measurements of tissue oxygenation. Free Radic. Biol. Med. 32, 139-147 (2002).

Ilangovan, G., Li, H., Zweier, J. L., Krishna, M. C., Mitchell, J. B., and Kuppusamy, P. In vivo measurement of regional oxygenation and imaging of redox status in RIF-1 murine tumor: Effect of carbogen-breathing. Magn. Reson. Med. 48, 723-7

Ilangovan, G., Pal, R., Zweier, J. L., and Kuppusamy, P. Electrochemical preparation and EPR studies of lithium phthalocyanine. Part 4: Effect of nitric oxide. J. Phys. Chem. B 106, 11929-11935 (2002).

Pandian, R. P., Parinandi, N. L., Ilangovan, G., Zweier, J. L., and Kuppusamy, P. Novel particulate spin probe for targeted determination of oxygen in cells and tissues. Free Radic. Biol. Med. 35, 1138-1148 (2003).

Rizzi, C., Samouilov, A., Kutala, V., Parinandi, N. L., Zweier, J. L., and Kuppusamy, P. Application of a trityl-based radical for measuring superoxide. Free Radic. Biol. Med. 35, 1608-1618(2003).

Pandian, R. P., Kutala, V. K., Parinandi, N. L., Zweier, J. L., and Kuppusamy, P. Measurement of oxygen consumption in mouse aortic endothelial cells using a microparticulate oximetry probe. Arch. Biochem. Biophys. 420, 169-175 (2003).

Ilangovan, G., Liebgott, T., Kutala, V. K., Petryakov, S., Zweier, J. L., and Kuppusamy, P. EPR oximetry in the beating heart: Myocardial oxygen consumption rate as an index of postischemic recovery. Magn. Reson. Med. 51, 835-842 (2004).

Ilangovan, G., Zweier, J. L., & Kuppusamy, P. Mechanism of oxygen-induced EPR line broadening in lithium phthalocyanine microcrystals. J. Magn. Reson. 170, 42-48 (2004).

Khramtsov, V.V, Grigor'ev, I.A., Foster, M.A., Lurie, D.J., Zweier J.L. and Kuppusamy, P. Spin pH and SH probes: enhancing functionality of EPR-based techniques, Journal of Spectroscopy, 18, 213-225 (2004).

Kutala, V. K., Parinandi, N., Zweier, J. L., and Kuppusamy, P. Reaction of superoxide with trityl radical: Implications for the determination of superoxide by Spectrophotometry. Arch. Biochem. Biophys. 424, 81-88 (2004).

Ilangovan, G., Bratasz, A., Li, H., Schmalbrock, P., Zweier, J. L, Kuppusamy, P. In vivo measurement and imaging of tumor oxygenation using coembedded paramagnetic particulates. Magn. Reson. Med. 52, 650-657 (2004).

Ilangovan, G., Osinbowale, S., Bratasz, A., Bonar, M., Cardounel, A. J., Zweier, J. L., and Kuppusamy, P. Heat-shock attenuates respiration of cardiomyocytes through upregulation of nitric oxide synthase. Am. J. Physiol. Cell Physiol. 287, C1472-1481 (2004).

Kuppusamy, P. and Zweier, J. L. Cardiac applications of EPR imaging. NMR in Biomedicine, 17, 226-239 (2004).